In order to further explore the potential value of serum 25(OH)VD and T-VDR to establish the diagnosis of DFO, the levels of serum 25(OH)VD and T-VDR expression were evaluated in 156 cases peripheral blood samples and wound margin tissue samples in independent groups; these samples included samples of 72 patients with DFU without osteomyelitis and of 84 patients with DFO. Here, VDR is linked to osteomyelitis.