CMTM7 and breast cancer: Finally, RT-qPCR (Fig. 1I) and western blot (Fig. 1J) were conducted to detect CMTM7 mRNA and protein levels in breast cell lines, including normal breast cell line MCF10A and breast cancer cell lines MDA-MB-231, CAL-51, SKBR3, MCF-7, and T47D, indicating that CMTM7 was decreased in all breast cancer cell lines as compared to MCF10A cells.