SMAD4 and Hepatic fibrosis: The progression and regression of hepatic fibrosis rely on a complex interplay of the hepatic microenvironment [42], to further explore whether TPLR inhibit the TGF-β/Smad signaling pathway under the internal environment, we examined the antifibrotic effect of TPLR on the CCl4-induced fibrotic mice, eight weeks after the repetitive injection of CCl4, proteins of Smad2/3 and Smad4 were significantly expressed, and Smad7 was obviously decreased in the model group.