Here, the polyphenolic glycosides extract of L. rotata (TPLR) was purified with the selectivity adsorption resin, and was analyzed by UPLC-Q/TOF/MSn, experiments were designed to investigate whether TPLR could ameliorate hepatic fibrosis and promote the apoptosis of HSCs by inhibiting the TGF-β/Smad signaling pathway in vivo and in vitro. Here, TGFB1 is linked to Hepatic fibrosis.