Further analysis of the external dataset from Memorial Sloan Kettering Cancer Center (MSKCC) database including 137 GIST patients with 29 of them being wt-GIST confirmed that most frequently altered genes were NF1, EGFR, tumor protein p53(TP53), Succinate Dehydrogenase Complex Flavoprotein Subunit A(SDHA), Retinoblastomal 1(RB1), BRAF, Succinate Dehydrogenase Complex Flavoprotein Subunit B(SDHB), and TSC2, as shown in Fig. 4C. The analysis of KEGG pathways suggested that actin cytoskeleton, longevity regulating pathway and FoxO pathway indeed more frequently existed in the wt-GISTs. This evidence concerns the gene TP53 and gastrointestinal stromal tumor.