We observed that the splicing differences of RPS20-exon1-AP, KIF13B-exon4-SE and UBE2B-exon7-SE between the TB and HC groups mainly came from the “20–40 years” (ΔPSI = −0.07, P < 0.001; ΔPSI = 0.14, P < 0.001; ΔPSI = 0.11, P < 0.001) and “40–60 years” subgroups (ΔPSI = −0.04, P = 0.003; ΔPSI = 0.11, P < 0.001; ΔPSI = 0.12, P < 0.001), but not the “<20 years” subgroup. Here, RPS20 is linked to tuberculosis.