However, given our finding that in AP, there was a nonparallel change in the levels of p-MLKL and p-RIPK3, we further explored whether the expression of p-MLKL is independent of Ripk3. We constructed AP models in WT and Ripk3-/- mice, and the results of immunohistochemistry (Fig. 1H and Supplementary Fig. 1G) and western blot (Fig. 1I and Supplementary Fig. 1H) showed that there was a significant upregulation of p-MLKL in the pancreas from both WT mice and Ripk3-/- mice with AP. Here, MLKL is linked to alkaline phosphatase measurement.