Two novel ACE-1 inhibitory peptides with amino acid sequences corresponding to IGNNPAKGGLF and YIGNNPAKGGLF were identified from a 3 kDa permeate of a protein hydrolysate generated from the brown seaweed L. digitata. In silico methods also predicted the potential of this seaweed as a source of novel, bioactive peptides that may impart additional health benefits to the consumer including prevention of T2D and antimicrobial activities following GI digestion. This evidence concerns the gene ACE and type 2 diabetes mellitus.