Among 55 DEmRNA, higher expressions of 19 mRNA, namely CPEB3, EFNB3, FATA4, growth hormone receptor (GHR), GSTZ1, KLF8, MFAP4, PAIP2B, PHACTR3, PITPNM3, RPS6KA6, RSPO3, SLITRK6, SMOC1, STEAP4, TMEM132E, TSPAN11, and ZFPM2 were associated with longer OS in 364 HCC patients (P < .05) (Fig. 4). The gene discussed is RSPO3; the disease is hepatocellular carcinoma.