The presence of gallstones was significantly correlated among cancer cases and inflammatory lesions (p < 0.0001).The receiver operating characteristic (ROC) curve was plotted to determine the optimum PDL1 concentration for predicting PDL1 expression level (high/low).The area under the curve obtained was 0.5053 [95% confidence interval (95%CI): 0.2773 to 0.7333, p = 0.9634]. The gene discussed is CD274; the disease is gallstones.