MAP1LC3A and Alzheimer disease: Finally, inhibiting neuronal PIKfyve activity significantly increased the number of LC3-positive autophagic puncta, which is in concordance with the presence of various types of electron-dense autophagic vesicles (AVs) reported in post-mortem neocortex and hippocampus of AD cases (Boland et al., 2008; Bordi et al., 2016; Tang et al., 2015).