In order to further the prognostic factor for OS of cholangiocarcinoma patients, we used the Cox univariate regression model to analyze the effects of clinical characteristics on OS, and results showed that the levels of CA199 and CEA, TNM stage, nerve invasion, postoperative treatment, preoperative cholangitis, pathological type, AST, ALB, and LMR all significantly affected the OS (Table 3). This evidence concerns the gene CEACAM5 and cholangiocarcinoma.