Only IL-18 production but not IFNs or CXCL1 were virtually disturbed in Casp1-KO Caco-2-ACE2-N IECs upon SARS-CoV-2 GFP/ΔN-trVLP infection, demonstrating that NLRP6-caspase1 axis is responsible for IL18 regulation in Caco-2-ACE2-N IECs (Figure 5C). Here, ACE2 is linked to infection.