In the context of SARS-CoV-2 GFP/ΔN-trVLP infection, cytokine production of IFNλ2/3, IL-18 (Figure 5C) was reduced in NLRP6-knockout Caco-2-ACE2-N IECs compared with parallel control either at mRNA or protein level, suggesting a pivotal role of NLRP6 in regulating SARS-CoV-2 immunity and induction of IFNλ2/3, IL-18 cytokine. This evidence concerns the gene ACE2 and infection.