To determine whether NLRP6 engages in the regulation of SARS-CoV-2 immunity in IECs, we established NLRP6 knockout cell line in basis of Caco-2-ACE2-N IECs and evaluated the cytokine production with SARS-CoV-2 GFP/ΔN-trVLP infection. This evidence concerns the gene ACE2 and infection.