To further understand the biological connection between PTEN and Entpd5/IGF1R pathway, we first assessed the expression levels of ATF6 in melanoma cells carrying the various PTEN mutants by western blot (Figure 8A); ATF6 expression was reduced in PTEN WT cells, but enhanced in PTEN ΔLP cells. The gene discussed is ENTPD5; the disease is melanoma.