To further elucidate the role of Entpd5 in PTEN-suppressed metastasis, Entpd5 was overexpressed in poorly metastatic mouse B16F1-PTEN WT and human melanoma A375p cells, where expression levels of Entpd5 are relatively low and PTEN relatively high (Figure 5C-a). This evidence concerns the gene PTEN and melanoma.