GPC3 and hepatocellular carcinoma: Previous studies have confirmed that the liver iron content of fibrotic livers is higher than that of normal liver, and the iron content of GPC-3-positive HCC patients is higher than that of GPC-3-negative patients, which may be related to the overexpression of transferrin receptors on the surface of HCC that cause a massive deposition of iron in the liver (21, 34, 35).