Having established that i.n. Tri:ChAd:TB induces long-lasting multifunctional antigen-specific CD8+ T cells within the LPT, we next profiled the expression of tissue-resident memory surface markers CD69, CD103, and CD49a59,60 by Ag85A-, TB10.4- and RpfB-specific CD8+IFNγ+ T cells using t-SNE analysis on concatenated CD3+CD8+CD4− BAL mononuclear cells from Tri:ChAd:TB-immunized animals (Fig. 1I, left panel). This evidence concerns the gene CD69 and tuberculosis.