By expressing Ag85A, TB10.4, and RpfB, animals immunized with Tri:ChAd:TB had significantly reduced pulmonary mycobacterial burden which was associated with markedly reduced lung immunopathology in comparison to animals immunized with the vaccines that lacked the antigenic breadth or did not span the spectrum of the M.tb life cycle (Fig. 3). The gene discussed is TMSB10; the disease is tuberculosis.