To analyze TAM activation and cell metabolic state in response to CD40 stimulation, we engrafted the YUMM1.7 melanoma cell line20 into wild-type mice followed by either control vehicle (phosphate-buffered saline, PBS) or agonistic anti-CD40 monoclonal antibody (FGK45) treatments (Extended Data Fig. 1a). Here, CD40 is linked to melanoma.