Of note, characterization of the genotypes of the four different organoid lines used throughout this study revealed all donors to not be carriers of three well-established coding NAFLD risk SNPs (PNPLA3 I148M, TM6SF2 E167K and GCKR P446L), with the exception of one donor being a heterozygous carrier of GCKR P446L (Supplementary Fig. 1c and Supplementary Table 1). The gene discussed is PNPLA3; the disease is metabolic dysfunction-associated steatotic liver disease.