The combination of metformin with the protein kinase inhibitor, sorafenib, suppressed EMT and significantly minimized the postoperative recurrence and lung metastasis of hepatocellular carcinoma in an orthotopic mouse model [254], and induced coiled-coil domain containing 65 (CCDC65) expression to suppress alpha-enolase (ENO1)-AKT1 pathway-mediated EMT in gastric cancer cells [255]. This evidence concerns the gene ENO1 and gastric cancer.