Therefore, in the present study, we analyzed the molecular mechanism of OPG in NASH by knocking out TNFRSF11B in vivo, combined with an animal model of NASH induced by a methionine- and choline-deficient (MCD) diet, to provide data for identifying promising targets for NASH treatment. Here, TNFRSF11B is linked to metabolic dysfunction-associated steatohepatitis.