To determine whether this system can be adopted to improve the efficacy of ClearColi-mediated gene editing in KCs, we constructed a dual-sgRNA CRISPR/CasΦ vector (29) encoding CasΦ-2 nuclease and 2 Crig-targeting sgRNAs (pPP441-2U6-2sgCrig) (Supplemental Figure 5C). Here, VSIG4 is linked to dry eye syndrome.