The assessment of immune infiltration is complex, encompassing multiple immune cell types, and studies have shown that immune infiltration has significant value in the prognosis of tumor patients (such as colorectal cancer, hepatocellular pancreatic and gallbladder carcinoma) and their response to therapy.[55] In our study, 6 types of immune cells were included: B cells, T cells (CD4), T cells (CD8), neutrophils, macrophages, and dendritic cells. The gene discussed is CD8A; the disease is colorectal cancer.