To estimate the mechanism of USP21 promoting the migration, invasion, and stemness profiles of nasopharyngeal carcinoma cells by the FOXM1 pathway, western blot assay was used to check the expression of FOXM1 in nasopharyngeal carcinoma cells after sh-USP21#1 and sh-USP21#2 transfection. This evidence concerns the gene FOXM1 and nasopharyngeal carcinoma.