Further, the RNF38 gene expression decreased in the intended comparison by representing a significant difference regarding its expression level between the males with asthenozoospermia and that of normal individuals in subgroups A and B. Furthermore, a significant difference was reported between the expression of this gene in the males with asthenozoospermia and that of the males with normozoospermia, its relationship with increasing P53 gene expression, and consequently a decrease in sperm motility (p < 0.001). This evidence concerns the gene RNF38 and Reduced sperm motility.