Here, the Western Blot results revealed that knockdown of EPS8L3 can markedly reduce the GSK3B expression in PC cells; and the recovery experimentations also suggested that overexpression of GSK3B can reversal the prohibitive effect of silencing EPS8L3 on the proliferative ability and metastasis of PC cells. This evidence concerns the gene EPS8L3 and pachyonychia congenita.