To validate the reliability of our RNA-Seq data, we selected three key DEmRNAs (NCOR2, ERBB3, and NOTCH1), three key DElncRNAs (NEAT1, KCNQ1OT1, and FGD5-AS1), and three key DEcircRNAs (hsa_circ_0087493, hsa_circ_0102923, and hsa_circ_0089902) for RT-qPCR analysis on AD-EVs and NC-EVs. The gene discussed is KCNQ1OT1; the disease is Alzheimer disease.