Interestingly, they also found liver-specific elevation in the levels of malonylated lysine and proteins in the liver tissues of db/db and ob/ob mice (both being typical models of type 2 diabetes [46, 47]) compared to those in wild-type (WT) mice; five proteins with increased lysine malonylation levels, namely glucose-6-phosphate isomerase (G6PI), 10-formyl-tetrahydrofolate dehydrogenase (10-FTHFDH), lactate dehydrogenase A (LDHA), fructose bisphos-phatase 1 (FBP1), and aldolase B (ALDOB), were further validated using western blot and immunoprecipitation analyses. This evidence concerns the gene LDHA and type 2 diabetes mellitus.