Moreover, transcript levels of markers of cardiac hypertrophy and dysfunction were assessed by RT-PCR in the isolated myocytes, which showed increased transcript levels of Nppa, Nppb, and Myh7 and decreased levels of Myh6 and Atp2a2 transcripts, all consistent with cardiac hypertrophy and dysfunction [Figure 6F]. This evidence concerns the gene NPPA and cardiac hypertrophy.