Transcript levels of over a dozen genes involved in myocardial fibrosis, namely Col1a1, Col3a1, Tgfb1, Tgfb2, Tgfb3, Ctgf, Postn, Mmp2, Mmp14, Timp1, Mgp, Lrp1, and Vim were analyzed by RT-PCR in independent samples from those used in the RNA-Seq, which showed marked increase in the levels of all selected genes in the Myh6-McmTam:DspF/F myocytes compared to the corresponding levels in the WT or Myh6-Mcm mice [Figure 5H]. This evidence concerns the gene TGFB1 and Myocardial fibrosis.