The heatmap showed that the B4 cluster expressed a series of lipid-metabolism-associated genes, such as APOA1, APOA2, and APOA3 (Figure 7D), and UMAP plotting also showed that B4 cells highly expressed APOC1 and APOE (Figure 8A), implying that the B4 cluster may be associated with steatosis in the ALC group. The gene discussed is APOA1; the disease is steatosis.