RiskScore of cholangiocarcinoma patients with 10 genes calculated followed the above formula: The RiskScore=0.429*VEGFC -0.434*NFKBIA-0.884*NLRX1+0.54*AKT1+0.629*CSRP1+0.406*EPO+0.833*IL31RA+1.023*LEP+0.341*MUC4+0.363*SEMA4B. The gene discussed is MUC4; the disease is cholangiocarcinoma.