And it was reported that specific nAChRs seem to have different roles in the effects of nicotine on anxiety, for example, α7 and α4β2 nAChRs differentially modulate both anxiety and nicotine’s effects on anxiety (Collo, et al., 2018; Oni, et al., 2016; Kutlu, M.G, and Gould, T.J, 2015). This evidence concerns the gene IGKV2D-24 and Anxiety.