MAPK and related kinase pathways are associated with neurodegenerative diseases including PD, and MAPK signaling pathway plays an important role in cell proliferation apoptosis, differentiation and inflammatory responses, etc. According to previous studies, the mammalian MAPK signaling pathway protein markers (JNK, ERK, p38) were investigated by JESS system to confirm whether Nic achieves neuroprotective effects in MPTP-induced PD mice by modulating the pathway. The gene discussed is MAPK8; the disease is neurodegenerative disease.