TNNI3 and neoplasm: As a future direction, a survival study could be performed where each animal will be treated until euthanasia criteria are met, including tumor volumes, body weights, etc. Also, the current study has not assessed whether the RCM1/VCR combination therapy could be universally effective for the different subtypes of RMS, including embryonal, alveolar, pleomorphic, and spindle cell/sclerosing variants.