PHC3 and neoplasm: Immunostaining of Rd76-9 cells for CHAC1, Ki-67, PH3 and Cleaved-Caspase 3 protein demonstrated that the depletion of CHAC1 decreased the numbers of Ki-67+ and PH3+ proliferating tumor cells (Figures 6D-F) as well as increased the number of tumor cells undergoing apoptosis (Figure 6G).