The validation cohort showed GPC3 was negatively correlated with all liver fibrosis markers ACTA2, COL1A1, COL1A2, COL3A1 and ductular reaction markers KRT7 and KRT19, while SDC4 was positively correlated with ductular reaction index KRT19 (Supplementary Figure S6). The gene discussed is COL1A1; the disease is Hepatic fibrosis.