OBSCN and hepatocellular carcinoma: In our cohort (N = 125), the frequencies of mutations in these genes were equally high, predominantly with missense mutations, and TTN was the gene with the highest mutation frequency; other genes with higher mutation frequencies were as follows: TP53 (30%), OBSCN (23%), APOB (22%), ADGRV1 (22%), etc. The mutation frequency was altered compared to the somatic mutation data for HCC in the TCGA and ICGC databases (Figure 1E).