To further validate the findings in vitro, after tumor homogenization, we determined the ERS-related proteins Grp78, CHOP, and the BTG3/PI3K/AKT pathway-related proteins BTG3, PI3K, AKT, p-PI3K, and p-AKT using Western blot, which revealed that BTG3 was remarkably elevated and the levels of p-PI3K and p-AKT were diminished in nude mice treated with 50 mg/kg/d baicalein compared with the 15 mg/kg/d baicalein group (Fig. 6E, F, all p < 0.001). This evidence concerns the gene HSPA5 and neoplasm.