Taken all together, although it was hard to notice any substantial difference in spiking patterns of individual RGCs in the rd8 retinas (minimal difference in ON RGCs and almost no difference in OFF and DS RGCs; see Figures 4B, C) compared to those of the wt retinas (Lee and Im, 2018, 2019; Yoon et al., 2020; Otgondemberel et al., 2021), their populational characteristics appeared to be markedly altered in OFF and DS pathways (Figures 5, 6A) even with the early progression of retinal degeneration caused by Crb1 mutation. Here, CRB1 is linked to retinal degeneration.