Further analysis for the correlation between the expression level of E2F8 and immune cell subtypes, including those in breast cancers, revealed that the expression level of E2F8 was markedly positively related to these immune cells, especially CD8+ T cells, CD4+ T cells, neutrophil, and dendritic cells in the basal-like subtype, while the correlation between the expression level of MYBL2 and immune cells was not obviously exhibited in the basal-like subtype (Supplementary Figure 5). The gene discussed is E2F8; the disease is breast cancer.