In conclusion, GRIM-19 is closely related to the occurrence of asthenozoospermia, GRIM-19 overexpression promotes GC-2 spd cell proliferation and migration and reduces apoptosis, while GRIM-19-silenced reduces GC-2 spd cell proliferation and migration and increased apoptosis. The gene discussed is NDUFA13; the disease is Reduced sperm motility.