GAPDH and Reduced sperm motility: Western blotting analysis showed that the expression of protein of GRIM-19 in the spermatozoa of patients with asthenozoospermia was also significantly lower than in the normal control group (GRIM-19/GAPDH: 0.827 ± 0.063 vs 0.458 ± 0.033; P < 0.001) (Fig. 2B).