GRIM-19 has an important role in cell electron transfer activities, studies have demonstrated down-regulation of GRIM-19 leads to the breakdown of mitochondrial membrane potential and increased reactive oxygen species (ROS) levels15,16, changes in ROS concentrations are closely associated with sperm motility, energy acquisition and acrosome reactions, which may suggest that GRIM-19 is closely related to the occurrence of asthenozoospermia. This evidence concerns the gene NDUFA13 and Reduced sperm motility.