TP53 and acute lymphoblastic leukemia: The differentially expressed genes (adjusted P-value < 0.05, Pre-T ALL vs. normal) in the T cell clusters are significantly enriched in 6 pathways, including apoptosis, cell cycle, cGMP−PKG signaling, NF − kappa B signaling, p53 signaling, and T cell receptor signaling pathways (Fig. 5c).