We used a viral infection to overexpress TNFR2 via encapsulation of the CSIV-TRE-RfA-CMV-KT-TNFR2 plasmid with a Flag tag in K562 and K562/ADR cell lines, which were treated with or without BS3 for the cross-linking of cell-surface proteins. This evidence concerns the gene TNFRSF1B and viral infectious disease.