MLANA and melanoma: To ensure specific and equal recognition by MART-1 T cells and exclude potential confounding effects of differences in IFNγ signaling, antigen presentation machinery, and major histocompatibility complex (MHC) and antigen expression levels, we ectopically expressed through lentiviral transduction HLA-A∗02:01 and MART-1 in a panel of human melanoma cell lines, including patient-derived melanoma xenograft (PDX) cell lines.17