Notably, the second patient identified in this study (Case 2) is also hemizygous for a nonsense NLGN3 variant and, although prepubertal, displayed typical red flag signs of hypogonadism, including small testes volume and micropenis (Boehm et al., 2015; Swee and Quinton, 2019). This evidence concerns the gene NLGN3 and hypogonadism.