Similarly in BT474 cells, there was a significant increase in cell population in G1 phase with shDVL2 + Nert combination compared to NTC and shDVL2 alone while significant reduction was observed in G2/M phase population with shDVL2 + Nert combination compared to NTC control (p < 0.05) indicating that the additive effect of DVL2 and HER2 inhibition against cancer cell proliferation might be more prominent in HER2-amplified cells which are hormone receptor negative not hormone receptor positive (ER/PR+) cells. This evidence concerns the gene ESR1 and cancer.