To this end, we first transfected Huh7 liver carcinoma cells to express either the LJ144 HBV genetic clone encoding wild-type assembly-competent Cp (hereafter referred to as “WT Cp”) or the J305 clone encoding Cp-Y132A co-expressed with LJ145 that encodes a Cp-minus packageable genome substrate (17, 33). Here, CP is linked to hepatocellular carcinoma.