Therefore, we isolated basal (Lin− CD24lo CD49fhi) and luminal CD61+ and CD61− (Lin− CD24hi CD49flo CD61±) cells from 2.2–2.6-week-old PyMT+/− and PyMT+/−Rank+/tg mice (before clonal tumor populations were detected) and implanted them orthotopically into the mammary fat pads of syngeneic FVB female mice. The gene discussed is ITGB3; the disease is neoplasm.