The expression level of Klotho was seen to be significantly increasing in quercetin-treated colon cancer cells (Fig. 4a), In HCT 116, the expression of Klotho was significantly increased in HQ80μM (***p = 0.001) and HQ120μM (***p < 0.001) compared to the HCT 116 control group. Here, KL is linked to malignant colon neoplasm.