In summary, our study found that 11 biomarkers (such as IL-8, IL-24, CCL20, OPG, TSLP, TRAIL, MMP-10, CXCL1, MCP-4, TNFSF14, and LIF) have high values in identifying NEC and determining the severity of NEC. This evidence concerns the gene IL24 and necrotizing enterocolitis.