Furthermore, we identified scaRNAs that are differentially expressed in FUS KO cells and some pseudouridylation sites in snRNAs that were altered in these cells and in FUS R495X mutant (Supplementary Table S1, Supplementary Fig. S5B) which could partially explain RNA splicing defects reported in FUS depleted cells and caused by ALS-associated FUS mutations33–35. Here, FUS is linked to amyotrophic lateral sclerosis.