We used HEK293T and SH-SY5Y cells for our work as SH-SY5Y cells with FUS knockout and FUS R495X mutation offered a good initial model to recapitulate ALS cells, while HEK293T FUS KO offered a non-neuronal cell type to identify changes in rRNA modifications between two completely different cell lines with the same phenotype (FUS KO). Here, FUS is linked to amyotrophic lateral sclerosis.