SHMTs comprised two isozymes of SHMT2 and SHMT1 in cells, we performed bioinformatics analysis based on The Cancer Genome Atlas (TCGA) database and found that SHMT2, but not SHMT1, was overexpressed in cancer specimens, with an average 1.522-fold increase compared with controls (Fig. 1A). The gene discussed is SHMT2; the disease is cancer.