To validate the differential distribution of myeloid cell subtypes in other cancer types, we classified subtypes of myeloid cells according to the clustering of sub-spot GEPs in HCC tissues, including monocytes, TGFB1+ macrophages (Macro-TGFB1), Macro-SPP1, and MACRO+ macrophages (Macro-MARCO) (Fig. 4h, Supplementary Fig. 5i–l). This evidence concerns the gene TGFB1 and hepatocellular carcinoma.