Subgroup analysis showed that DNA methylation level over − 153 CpG site of the LC3B gene was further increased in SDB patients with EDS versus those without EDS (1.54 ± 0.96 versus 1.1 ± 0.74, adjusted p = 0.011, Fig. 3D), and was positively correlated with ESS (R = 0.327, p = 0.003, Fig. 3E). The gene discussed is MAP1LC3B; the disease is Ehlers-Danlos syndrome.